Thyroid cancer series (video 1/11) – Who is Dr. Karim Sarhane ?
سلسلة سرطان الغدة الدرقية – كل ما تريد معرفته عن عقيدات الغدة الدرقية (فيديو 1/11) – من هو الدكتور كريم؟
الدكتور كريم سرحان
استشاري الجراحة ورئيس القسم
الجراحة العامة والمناظير
دبلوم البورد الأمريكي للجراحة
زميل مشارك، كلية الجراحين الأمريكية
العين، أبو ظبي، الإمارات العربية المتحدة
شكرا لكم على المشاهدة، ونتمنى لكم دوام الصحة
Karim Ahmad Sarhane, MD MSc
Consultant Surgeon and Head of Department
General and Laparoscopic Surgery
Diplomate of the American Board of Surgery
Associate Fellow, American College of Surgeons
Al Ain, Abu Dhabi, UAE
Thank you for watching, and I wish you the best of health
https://burjeel.com/burjeelroyal/doctors/karim-ahmad-sarhane/ https://drkarim.com
Thyroid Cancer series – All what you need to know about Thyroid Nodules (video 1/11) – Who is Dr. Karim ?
سلسلة سرطان الغدة الدرقية – كل ما تريد معرفته عن عقيدات الغدة الدرقية (فيديو 1/11) – من هو الدكتور كريم؟
الدكتور كريم سرحان
الجراحة العامة والمناظير
دبلوم البورد الأمريكي للجراحة
زميل مشارك، كلية الجراحين الأمريكية
العين، أبو ظبي، الإمارات العربية المتحدة
شكرا لكم على المشاهدة، ونتمنى لكم دوام الصحة
Karim Sarhane, MD MSc
Consultant Surgeon and Head of Department
General and Laparoscopic Surgery
Diplomate of the American Board of Surgery
Associate Fellow, American College of Surgeons
Al Ain, Abu Dhabi, UAE
Thank you for watching, and I wish you the best of health
Click on my Business Card
Karim Sarhane, MD
Consultant Surgeon and HOD
التوعية بسرطان المرارة والقنوات الصفراوية
الدكتور كريم سرحان
Gallbladder and bile ducts cancer awareness
Dr. Karim Sarhane
علاج عقيدات الغدة الدرقية
الدكتور كريم سرحان
Management of Thyroid nodules
Karim Sarhane MD
May, 2024

Karim Sarhane – Management of appendicitis and appendicular neoplasms.
Management of Abdominal Pain and Neoplasms
May, 2024

Nanoparticle Compositions for Sustained Delivery of Pro-regenerative Growth Factors for Nerve Repair, Preparation Processes of the Same, and Treatment Methods Using the Same
Karim Sarhane, MD

Global incidence rates for peripheral nerve injury (PNI) are not well aggregated. However, US data collected shows that 20 million Americans suffer from peripheral nerve injury caused by trauma and medical disorders. When a tension-free early repair is possible, microsurgical direct nerve repair with epineural sutures remains the gold standard of care. The current standard for large nerve defects is autologous nerve grafts. However, disadvantages for this treatment approach include risk of neuroma formation and loss of donor nerve function. Acellular nerve conduits limit the disadvantages associated with autologous grafts. However, conduits are still insufficient for large deficits and require a combination of pharmacological and molecular therapies to yield prime results. Research indicates that these therapy additions should focus on both axonal regeneration and Schwann cell renewal. Insulin-like growth factor (IGF-1) has shown promising results because it optimizes axonal regeneration and Schwann cell renewal simultaneously. Previously reported encapsulation methods for growth factors either release the payload too rapidly or achieve prolonged presence through covalent conjugation. Therefore, there is a strong need to develop a delivery system that can provide sustained release of small proteins for an extended duration while maintaining encapsulation efficiency and bioactivity.
Karim Sarhane MD at Johns Hopkins created a nanoparticle-based delivery system that provides sustained release of bioactive insulin like growth-factor 1 (IGF-1) for 20 days in vitro to denervated nerve and muscle tissue within the peripheral nervous system. The delivery system fulfills the need to create a nanoparticle-based drug delivery system that provides sustained and controllable release of IGF-1 without sacrificing on encapsulation efficiency or retention of bioactivity. Preliminary results support potential to improve functional outcomes for PNI patients suffering from severe injury, presenting a significant value proposition with potential to improve significant sensory and motor impairments associated with 4th and 5th degree injury.

https://jhu.technologypublisher.com/technology/46035
Selectively permeable nanofiber construct for nerve repair and limb transplants

Karim Sarhane, MD
Purpose:
Substantial advances have been made in enhancing nerve regeneration across gaps through the use of conduits and acellular nerve grafts. However, very few therapeutic approaches have been successfully studied in primary end-to-end repairs. Post-repair histologic studies commonly demonstrate scar tissue between coapted nerve stumps. In this study, we propose a novel semi-permeable nanofiber nerve wrap prepared from FDA approved biocompatible materials (polycaprolactone) to reduce inflammation at nerve coaptation site through inhibition of inflammatory cell infiltration while allowing diffusion of essential nutrients and growth factors.
Methods
Nerve wraps were synthesized by electrospinning of randomly oriented 650-nm nanofibers, and constructs with pores smaller than 10 μm were obtained. Using Thy-1 GFP Sprague-Dawley rats, we performed sciatic nerve transection and epinureal repair (control group) and with wrapping the coaptation site using the neuro-protective nanofiber construct (experimental group). Five weeks later, histologic analysis (Masson’s Trichrome staining, ED1+TUJ1 immunofluorescence co-staining) was performed on nerve sections at the repair site to assess fibrosis (collagen deposition) and
inflammation (macrophage invasion) (n=5/group). Additionally, retrograde labeling was performed, and at the same time, the distal stump was harvested for histo-morphometric evaluation (n=8/group).
Results: Masson’s Trichrome and double immunofluorescence staining (ED1+TUJ1) of nerve longitudinal sections 5 weeks following repair showed a significantly decreased level of intraneural scarring and inflammation in the nanofiber nerve wrap group, as determined by collagen quantification (7.4% ± 1.3 vs. 3.2% ± 1.3, p<0.05) and macrophage counting (32.2 ± 2.4 cells/mm2 vs. 14.6 ± 1.8 cells/mm2, p<0.05) in the repair site. Collagen was trapped outside the nerve wrap in the experimental group. Nerve cross sections taken 5 mm distal to the coaptation site demonstrated a significantly increased number of myelinated axons in the experimental group. Retro- grade labeling showed a trend towards higher number of sensory dorsal root ganglion neurons that regenerated their axons in the nanofiber wrap group when compared to control.
Conclusion
These results provide new insights into a novel targeted anti-inflammatory approach in peripheral nerve repair. Electrospun nanofiber nerve wrap constructs protect the coaptation site from inflammation, promoting scar-free nerve repair, and enhancing axonal regeneration. This new therapeutic strategy utilizing FDA approved products holds great translational potential.
